The Absence of the TranscriptionFactor Yrr1p, Identified fromComparative Genome Profiling,Increased Vanillin Tolerance Due toEnhancements of ABC TransportersExpressing, r RNA Processing andRibosome Biogenesis inSaccharomyces cerevisiae

Scientific Achievement

In this work,new elements were found to increase resistance to vanillin including four oxidordeuctases, ABC transporters and the absence of transcription factor Yrr1p. And the new founction and mechanism of Yrr1p that its absence increase Saccharomyces cerevisiae resistance to vanillin were studied. These new findings lay the theoretical foundation to increase Saccharomyces cerevisiae resistance to lignocellulosic hydrolysates via genetic engineering technology.

 Research details

1.Four oxidordeuctases were confirmed to make contributions to vanillin resistance,two (Ald6p and Zwf1p) of which provide NADPH to vanillin reduction and two (YNL134C and YJR096W）have vanillin reduction activities.

 2.The multiple drug resistance related transcription factor gene YRR1 were identified to have InDel mutation and lose its protein function via comparative genome profiling. The absence of Yrr1p were confirmed to increase vanillin resistance due to enhancements of ABC transporters expressing, rRNA processing and ribosome biogenesis in Saccharomyces cerevisiae.